FL 1, BS, Q35

NS_Tutor_Katelyn
Posts: 136
Joined: Wed May 30, 2018 11:51 am
Location: Minnesota
Contact:

FL 1, BS, Q35

Postby NS_Tutor_Katelyn » Sun Aug 12, 2018 2:06 pm

A student asked us: "What do the pH markers on the figure indicate? I understand that migration of the proteins depends upon the interaction between their charge, and the charges of the anode and cathode, but I fail to understand the purpose of the pH's given."

In an electrophoretic gel molecules migrate based on their charge: those with a positive charge will move towards the cathode, those with a negative charge will move towards the anode. The charge of a protein changes based on the pH, so if you add a pH gradient to a gel then proteins will migrate towards the pH where they have a charge of zero, i.e. their isoelectric point.
Katelyn Sawyer
Senior Tutor
katelyn@nextsteptestprep.com

Return to “Post Questions About Exams and QBank”